Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 68,
  • Issue 1,
  • pp. 57-68
  • (2014)

Infrared and Raman Imaging for Characterizing Complex Biological Materials: A Comparative Morpho-Spectroscopic Study of Colon Tissue

Not Accessible

Your library or personal account may give you access

Abstract

Complementary diagnostic methods to conventional histopathology are currently being investigated for developing rapid and objective molecular-level understanding of various disorders, especially cancers. Spectral histopathology using vibrational spectroscopic imaging has been put in the frontline as potentially promising in this regard as it provides a “spectral fingerprint” of the biochemical composition of cells and tissues. In order to ascertain the feasible conditions of vibrational spectroscopic methods for tissue-imaging analysis, vibrational multimodal imaging (infrared transmission, infrared-attenuated total reflection, and Raman imaging) of the same colon tissue has been implemented. The spectral images acquired were subjected to multivariate clustering analysis in order to identify on a molecular level the constituent histological organization of the colon tissue such as the epithelium, connective tissue, etc., by comparing the cluster images with the histological reference images. Based on this study, a comparative analysis of important factors involved in the vibrational multimodal imaging approaches such as image resolution, time constraints, their advantages and limitations, and their applicability to biological tissues has been carried out. Out of the three different vibrational imaging modalities tested, infrared-attenuated total reflection mode of imaging appears to provide a good compromise between the tissue histology and the time constraints in achieving similar image contrast to that of Raman imaging at an approximately 33-fold faster measurement time. The present study demonstrates the advantages, the limitations of the important parameters involved in vibrational multimodal imaging approaches, and their potential application toward imaging of biological tissues.

PDF Article
More Like This
Hybrid confocal Raman endomicroscopy for morpho-chemical tissue characterization

Conor C. Horgan, Magnus Jensen, Ciro Chiappini, Tom Vercauteren, Richard Cook, and Mads S. Bergholt
Biomed. Opt. Express 13(4) 2278-2285 (2022)

Multiphoton morpho-functional imaging of healthy colon mucosa, adenomatous polyp and adenocarcinoma

Riccardo Cicchi, Alessandro Sturiale, Gabriella Nesi, Dimitrios Kapsokalyvas, Giovanni Alemanno, Francesco Tonelli, and Francesco S. Pavone
Biomed. Opt. Express 4(7) 1204-1213 (2013)

Multi-modal approach using Raman spectroscopy and optical coherence tomography for the discrimination of colonic adenocarcinoma from normal colon

Praveen C. Ashok, Bavishna B. Praveen, Nicola Bellini, Andrew Riches, Kishan Dholakia, and C. Simon Herrington
Biomed. Opt. Express 4(10) 2179-2186 (2013)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved