Multiple internal reflection spectroscopy has been applied to the study and identification of microgram quantities of pure steroids. The methodology described is simple and requires a minimum of sample preparation. The steroid spectra which have been obtained on 20 to 100 μg of sample are comparable to those obtained with KBr pellets. The steroids chosen to illustrate the method are of particular interest in assisting with the identification of the products of intermediary metabolism as it pertains to steroid hormones. The anomalous behavior of one of these steroids is discussed.
Eleanor L. Saier, "Use of Multiple Internal Reflection Spectroscopy in the Study and Identification of Steroids," Appl. Spectrosc. 22, 445-448 (1968)
References are not available for this paper.
OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.