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Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 38,
  • Issue 5,
  • pp. 697-700
  • (1984)

Infrared Spectrophotometric Determination of 13C Enrichment

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Abstract

A study has been made of the feasibility of using the <i>v</i><sub>3</sub> band of <sup>13</sup>CO<sub>2</sub> as a basis for measurement of the extent of isotope enrichment in breath tests. Correlation and least-squares treatments were used. Considering the constraints of a small computer, the correlation approach is the more satisfactory. The results indicate that a method that is based on a single measurement at 170 Torr, with a standard curve for quantitation, will, give results that are adequate, considering the natural variation that occurs. Assuming the use of a beam condenser, the amount of sample required is less than that normally taken for this application.

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