OSA's Digital Library

Applied Spectroscopy

Applied Spectroscopy

| PUBLISHED BY SAS — AVAILABLE FROM SAS AND OSA

  • Vol. 40, Iss. 1 — Jan. 1, 1986
  • pp: 106–108

Polarization Fluorometry to Determine Cell Density with Fluorochrome for DNA

Tsutomu Araki and Masaoki Yamada

Applied Spectroscopy, Vol. 40, Issue 1, pp. 106-108 (1986)


View Full Text Article

Acrobat PDF (337 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations
  • Export Citation/Save Click for help

Abstract

There is a need for a rapid and sensitive technique to estimate a small number of cells. The total number of both living and dead cells suspended in a solution can be measured directly with the use of the Coulter counter or a flow-cytometer, but both of these measurements are inaccurate for small bacteria, such as an Escherichia coli cell (0.6 μm diameter × 2 μm length). The bacterial density is determined by the measurement of the turbidity of a cell suspension with a nepherometer or, more conveniently, with a spectrophotometer. However, foreign particles (e.g., dust, protein, or lipid) often increase turbidity and result in an error in the assay. Fluorometric assay for DNA content provides a sensitive estimation of the cell density free from turbidity changes. For example, Hill and Whatley used an antibiotic drug (mithramycin) as a labeling fluorochrome to measure cell DNA density with a lower limit of 0.5 μg. Recently, more sensitive quantification of DNA has been achieved with a bibenzimidazole dye, Hoechst 33258 (H33258). This dye reacts with the adenine-thymine-rich region of DNA, resulting in a high quantum efficiency for the determination. Downs and Wilfinger applied H33258 to quantify DNA measurement, and they achieved detection limits as low as 4 ng for extracted DNA from pituitary cells.

Citation
Tsutomu Araki and Masaoki Yamada, "Polarization Fluorometry to Determine Cell Density with Fluorochrome for DNA," Appl. Spectrosc. 40, 106-108 (1986)
http://www.opticsinfobase.org/as/abstract.cfm?URI=as-40-1-106

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited