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Applied Spectroscopy

Applied Spectroscopy


  • Vol. 40, Iss. 1 — Jan. 1, 1986
  • pp: 106–108

Polarization Fluorometry to Determine Cell Density with Fluorochrome for DNA

Tsutomu Araki and Masaoki Yamada

Applied Spectroscopy, Vol. 40, Issue 1, pp. 106-108 (1986)

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There is a need for a rapid and sensitive technique to estimate a small number of cells. The total number of both living and dead cells suspended in a solution can be measured directly with the use of the Coulter counter or a flow-cytometer, but both of these measurements are inaccurate for small bacteria, such as an Escherichia coli cell (0.6 μm diameter × 2 μm length). The bacterial density is determined by the measurement of the turbidity of a cell suspension with a nepherometer or, more conveniently, with a spectrophotometer. However, foreign particles (e.g., dust, protein, or lipid) often increase turbidity and result in an error in the assay. Fluorometric assay for DNA content provides a sensitive estimation of the cell density free from turbidity changes. For example, Hill and Whatley used an antibiotic drug (mithramycin) as a labeling fluorochrome to measure cell DNA density with a lower limit of 0.5 μg. Recently, more sensitive quantification of DNA has been achieved with a bibenzimidazole dye, Hoechst 33258 (H33258). This dye reacts with the adenine-thymine-rich region of DNA, resulting in a high quantum efficiency for the determination. Downs and Wilfinger applied H33258 to quantify DNA measurement, and they achieved detection limits as low as 4 ng for extracted DNA from pituitary cells.

Tsutomu Araki and Masaoki Yamada, "Polarization Fluorometry to Determine Cell Density with Fluorochrome for DNA," Appl. Spectrosc. 40, 106-108 (1986)

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