Nuclei extracted from lymphocytes coming from normal subjects and from patients affected by chronic lymphatic leukemia (B-CLL) were studied by means of infrared spectroscopy. Substantial spectral differences were found above all in the region of the phosphate-group vibrations of DNA. The ratios of the integrated areas of the bands at 1080 and 1540 cm<sup>−1</sup>, due to the symmetrical stretching vibrations of PO<sub>2</sub><sup>−</sup> groups and to proteic components, respectively, assume increasing values, which are localized in quite separate ranges, for normal lymphocytes, leukemic lymphocytes, normal nuclei, and leukemic nuclei. These values, indicating a different distribution of DNA and of proteic components between normal and leukemic cells and between normal and leukemic nuclei, may assume a remarkable significance in the precocious diagnosis of leukemia (B-CLL) and in following the course of the disease. In the case of leukemic nuclei, the ratio, in weight, of the two components reaches the value of 1:1.
Enzo Benedetti, Maria Pia Palatresi, Piergiorgio Vergamini, Federico Papineschi, Maria Cristina Andreucci, and Giuliano Spremolla, "Infrared Characterization of Nuclei Isolated from Normal and Leukemic (B-CLL) Lymphocytes: Part III," Appl. Spectrosc. 40, 39-43 (1986)