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Applied Spectroscopy

Applied Spectroscopy

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  • Vol. 40, Iss. 5 — Jul. 1, 1986
  • pp: 673–680

Continuous, On-Line Interface for Reverse-Phase Microbore High-Performance Liquid Chromatography/Diffuse Reflectance Infrared Fourier Transform Analysis

M. A. Castles, L. V. Azarraga, and L. A. Carreira

Applied Spectroscopy, Vol. 40, Issue 5, pp. 673-680 (1986)


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Abstract

An ultrasonic nebulizer with a piezo-electric transducer and a newly designed and constructed continuous on-line collection station have been adapted as an interface between a reverse-phase microbore high-performance liquid chromatograph (RP-μHPLC) and a diffuse reflectance infrared Fourier transform spectrometer. The collection station incorporates a directional vacuum port for confinement of the nebulized material onto a small area (3 to 5 mm i.d.) of the diamond powder. The inherent desolvation that occurs upon nebulization of the reverse-phase eluant, along with the vacuum collection system and the diamond powder diffuse reflectance substrate, makes possible the continuous on-line collection and FT-IR analysis of the compounds separated by a RP-μHPLC without any type of initial solute extraction.

Citation
M. A. Castles, L. V. Azarraga, and L. A. Carreira, "Continuous, On-Line Interface for Reverse-Phase Microbore High-Performance Liquid Chromatography/Diffuse Reflectance Infrared Fourier Transform Analysis," Appl. Spectrosc. 40, 673-680 (1986)
http://www.opticsinfobase.org/as/abstract.cfm?URI=as-40-5-673


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