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Applied Spectroscopy

Applied Spectroscopy


  • Vol. 40, Iss. 8 — Nov. 1, 1986
  • pp: 1136–1141

Diode Array Spectrophotometry of Translucent Materials

William R. Englebreth, Charles K. Mann, and Thomas J. Vickers

Applied Spectroscopy, Vol. 40, Issue 8, pp. 1136-1141 (1986)

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The light scattered from translucent samples is used to obtain absorption-type spectra and provide quantitation of an absorber within the scattering bodies. Synthetic vesicles with an encapsulated dye, bromo-thymol blue (BTB), are employed as a novel model system to study the properties of translucent samples. Chlorophyll measurement in intact phytoplankton and algae is demonstrated. A least-squares fitting procedure is used to overcome the effect of chlorophyll fluorescence on the scattering data. A straight line relationship between a defined pseudo-absorbance and concentration is shown for both the BTB and chlorophyll data. A limit of detection of about 0.2 mg/L BTB is obtained for the synthetic vesicles. For chlorophyll the limit of detection is in the range of 1 to 5% of that found in the native plankton and algae samples.

William R. Englebreth, Charles K. Mann, and Thomas J. Vickers, "Diode Array Spectrophotometry of Translucent Materials," Appl. Spectrosc. 40, 1136-1141 (1986)

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