OSA's Digital Library

Applied Spectroscopy

Applied Spectroscopy

| PUBLISHED BY SAS — AVAILABLE FROM SAS AND OSA

  • Vol. 40, Iss. 8 — Nov. 1, 1986
  • pp: 1136–1141

Diode Array Spectrophotometry of Translucent Materials

William R. Englebreth, Charles K. Mann, and Thomas J. Vickers

Applied Spectroscopy, Vol. 40, Issue 8, pp. 1136-1141 (1986)


View Full Text Article

Acrobat PDF (600 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations
  • Export Citation/Save Click for help

Abstract

The light scattered from translucent samples is used to obtain absorption-type spectra and provide quantitation of an absorber within the scattering bodies. Synthetic vesicles with an encapsulated dye, bromo-thymol blue (BTB), are employed as a novel model system to study the properties of translucent samples. Chlorophyll measurement in intact phytoplankton and algae is demonstrated. A least-squares fitting procedure is used to overcome the effect of chlorophyll fluorescence on the scattering data. A straight line relationship between a defined pseudo-absorbance and concentration is shown for both the BTB and chlorophyll data. A limit of detection of about 0.2 mg/L BTB is obtained for the synthetic vesicles. For chlorophyll the limit of detection is in the range of 1 to 5% of that found in the native plankton and algae samples.

Citation
William R. Englebreth, Charles K. Mann, and Thomas J. Vickers, "Diode Array Spectrophotometry of Translucent Materials," Appl. Spectrosc. 40, 1136-1141 (1986)
http://www.opticsinfobase.org/as/abstract.cfm?URI=as-40-8-1136

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited