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Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 42,
  • Issue 1,
  • pp. 12-14
  • (1988)

Sensitivity Calibration of Multichannel Raman Spectrometers Using the Least-Squares-Fitted Fluorescence Spectrum of Quinine

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Abstract

A method of sensitivity calibration of multichannel Raman spectrometers is described which employs the fluorescence spectrum of quinine as an intensity standard. The reported absolute fluorescence spectrum of quinine was least-squares fitted with a polynomial so that it gave an interpolation of the spectrum in the "Raman scale." The obtained least-squares-fitted fluorescence spectrum was cross-checked with the rotational Raman spectrum of D<sub>2</sub>. The fluorescence intensities calculated for every 100 cm<sup>−1</sup> from the two Ar<sup>+</sup> laser lines are tabulated for convenience.

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