OSA's Digital Library

Applied Spectroscopy

Applied Spectroscopy

| PUBLISHED BY SAS — AVAILABLE FROM SAS AND OSA

  • Vol. 42, Iss. 1 — Jan. 1, 1988
  • pp: 138–141

In Situ Measurement of the FT-IR Spectra of Phospholipid Monolayers at the Air/Water Interface

Richard A. Dluhy, Norman A. Wright, and Peter R. Griffiths

Applied Spectroscopy, Vol. 42, Issue 1, pp. 138-141 (1988)


View Full Text Article

Acrobat PDF (410 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations
  • Export Citation/Save Click for help

Abstract

External reflection Fourier transform infrared spectroscopy has been applied to the in situ measurement of the infrared spectra of insoluble phospholipid monolayer films that were spread at the air/water interface. The spreading conditions for the phospholipids [1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC)] were chosen to mimic those of liquid-condensed and liquid-expanded surface films, respectively. It is shown that the infrared reflection spectra can identify vibrations due to the hydrocarbon acyl chains, carbonyl ester, and phosphate groups for these monolayer films on water. Detailed examination of the frequencies and polarization properties of the C-H stretching region shows that the liquid-condensed DSPC acyl chains are in a rigid, mostly all-trans configuration, while the liquid-expanded DMPC acyl chains are highly fluid. The spectra demonstrate that the external reflection FT-IR experiment can differentiate between the physical conformation of monomolecular films when water is used as a reflective substrate.

Citation
Richard A. Dluhy, Norman A. Wright, and Peter R. Griffiths, "In Situ Measurement of the FT-IR Spectra of Phospholipid Monolayers at the Air/Water Interface," Appl. Spectrosc. 42, 138-141 (1988)
http://www.opticsinfobase.org/as/abstract.cfm?URI=as-42-1-138

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited