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Applied Spectroscopy

Applied Spectroscopy


  • Vol. 42, Iss. 8 — Nov. 1, 1988
  • pp: 1365–1368

MAGIC-LC/FT-IR Spectrometry: Preliminary Studies

R. M. Robertson, J. A. de Haseth, J. D. Kirk, and R. F. Browner

Applied Spectroscopy, Vol. 42, Issue 8, pp. 1365-1368 (1988)

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A new solvent elimination interface based on the Monodisperse Aersol Generation Interface for Combining Liquid Chromatography with Fourier transform infrared (MAGIC-LC/FT-IR) spectrometry is described. The solvent elimination efficiency of MAGIC-LC/FT-IR was studied by varying the mobile-phase composition from 100% methanol to 100% water. As the mobile-phase composition was varied, erythrosin B was injected into the interface and deposited on a KBr window after the solvent removal. Spectra were obtained which compared favorably with reference spectra, even as the mobile-phase water content was increased. A reverse-phase separation was completed to demonstrate that readily identifiable spectra can be obtained from mobile phases containing high percentages of water, without heating of the effluent stream.

R. M. Robertson, J. A. de Haseth, J. D. Kirk, and R. F. Browner, "MAGIC-LC/FT-IR Spectrometry: Preliminary Studies," Appl. Spectrosc. 42, 1365-1368 (1988)

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