OSA's Digital Library

Applied Spectroscopy

Applied Spectroscopy

| PUBLISHED BY SAS — AVAILABLE FROM SAS AND OSA

  • Vol. 42, Iss. 8 — Nov. 1, 1988
  • pp: 1405–1412

The Steady-State and Decay Characteristics of Protein Tryptophan Fluorescence from Algae

M. Baek, W. H. Nelson, P. E. Hargraves, J. F. Tanguay, and S. L. Suib

Applied Spectroscopy, Vol. 42, Issue 8, pp. 1405-1412 (1988)


View Full Text Article

Acrobat PDF (965 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations
  • Export Citation/Save Click for help

Abstract

The intrinsic steady-state fluorescence due to tryptophan has been obtained from monospecific cultures of fourteen plankton algae of various genera. Fluorescence decay profiles of protein tryptophan residues were obtained for eight marine plankton algae. Each organism exhibits a strong maximum in its emission spectrum at 320-340 nm when excited at 290 nm. Iodide quenching and denaturization experiments with 8 M urea provide strong evidence for the assignment of the 320-340 nm fluorescence to protein tryptophan. Most importantly, the decay of this bacterial protein tryptophan fluorescence has been described. The observation that characteristic protein-tryptophan fluorescence lifetimes have been obtained for each organism suggests that measurements of fluorescence lifetimes may be helpful in the rapid characterization of algae. Direct application will likely be found in combination with the measurement of other luminescence parameters.

Citation
M. Baek, W. H. Nelson, P. E. Hargraves, J. F. Tanguay, and S. L. Suib, "The Steady-State and Decay Characteristics of Protein Tryptophan Fluorescence from Algae," Appl. Spectrosc. 42, 1405-1412 (1988)
http://www.opticsinfobase.org/as/abstract.cfm?URI=as-42-8-1405

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited