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Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 42,
  • Issue 8,
  • pp. 1469-1472
  • (1988)

High-Precision Fluorimetry with a Light-Emitting Diode Source

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Abstract

A simple fluorimeter which uses a light-emitting diode as a source of excitation and a photodiode as a detector has been constructed and evaluated. The exceptional stability of both the detector and the source make it possible to make concentration measurements by fluorimetry with a precision of at least 0.002%. Furthermore, the system exhibited excellent linear dynamic range and a limit of detection of 20 ng/L for the dye Oxazine 720 in methanol.

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