Automated Image Analysis for Distortion Compensation in Sequencing Gel Electrophoresis
Applied Spectroscopy, Vol. 46, Issue 1, pp. 136-141 (1992)
Acrobat PDF (5080 KB)
Abstract
A computerized method is described for correcting systematic distortion of images from slab gel electrophoresis. Such distortions can lead to misinterpretation of the information contained in the gel. The method is useful for data analysis in one-dimension slab gel electrophoresis where the information is manifested in rectangular shaped bands, such as conventional restriction digest or sequencing gels, and the distortions can be adequately described by continuous low-order polynomial functions. The purpose is to eliminate human skill and judgement from the process and to minimize human interaction, which would be useful in any future attempts to automate the analysis of electrophoretic gels.
Citation
Lance B. Koutny and Edward S. Yeung, "Automated Image Analysis for Distortion Compensation in Sequencing Gel Electrophoresis," Appl. Spectrosc. 46, 136-141 (1992)
http://www.opticsinfobase.org/as/abstract.cfm?URI=as-46-1-136
You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
« Previous Article | Next Article »
OSA is a member of 