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Applied Spectroscopy

Applied Spectroscopy

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  • Vol. 46, Iss. 1 — Jan. 1, 1992
  • pp: 136–141

Automated Image Analysis for Distortion Compensation in Sequencing Gel Electrophoresis

Lance B. Koutny and Edward S. Yeung

Applied Spectroscopy, Vol. 46, Issue 1, pp. 136-141 (1992)


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Abstract

A computerized method is described for correcting systematic distortion of images from slab gel electrophoresis. Such distortions can lead to misinterpretation of the information contained in the gel. The method is useful for data analysis in one-dimension slab gel electrophoresis where the information is manifested in rectangular shaped bands, such as conventional restriction digest or sequencing gels, and the distortions can be adequately described by continuous low-order polynomial functions. The purpose is to eliminate human skill and judgement from the process and to minimize human interaction, which would be useful in any future attempts to automate the analysis of electrophoretic gels.

Citation
Lance B. Koutny and Edward S. Yeung, "Automated Image Analysis for Distortion Compensation in Sequencing Gel Electrophoresis," Appl. Spectrosc. 46, 136-141 (1992)
http://www.opticsinfobase.org/as/abstract.cfm?URI=as-46-1-136

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