An analytical application for Surface-Enhanced Raman Spectroscopy at a silver electrode is described. Real-time SER spectra of adenine and cytosine have been recorded in a 10-μL spectroelectrochemical flow cell under flowing conditions. Charge-coupled-device detection allowed high-quality spectra spanning a ~1200 cm−1 region to be recorded with integration times of 4 seconds. A low-power He.Ne laser was used as a source. SERS at the silver electrode offers rapid time response to adsorption/desorption by appropriate potential modulation. The technique is extremely reproducible and insensitive to temperature and flow rate. The effects of incident photon energy and applied potential on the intensity of the Raman signal are discussed.
Neil J. Pothier and R. Ken Forcé, "Surface-Enhanced Raman Spectroscopy at a Silver Electrode as a Real-Time Detector in Flowing Streams," Appl. Spectrosc. 46, 147-151 (1992)