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Applied Spectroscopy

Applied Spectroscopy


  • Vol. 46, Iss. 1 — Jan. 1, 1992
  • pp: 169–175

Investigation of β-Cyclodextrin Immobilized at Silica Surfaces by Fluorescence Spectroscopy

Kevin S. Litwiler and Frank V. Bright

Applied Spectroscopy, Vol. 46, Issue 1, pp. 169-175 (1992)

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Steady-state and frequency-domain fluorescence spectroscopy are used to study the inclusion complexation of six anilinonaphthalene sulfonates (ANSs) with silica-immobilized beta-cyclodextrin (βCD). To fit the experimental data, we compared several modified Lorentzian lifetime distribution models. However, in all cases studied, a simple unimodal Lorentzian distribution best fit the fluorescence decay kinetics. The recovered distribution shapes did not change substantially as the surface was titrated with ANS; however, subtle trends suggest that silica-immobilized βCDs in more hydrophobic environments complex first. Furthermore, the recovered distribution widths (a measure of the environmental heterogeneity) for the silica-immobilized βCD-ANS complexes are always broader than those for ANS-βCD complexes in bulk solution.

Kevin S. Litwiler and Frank V. Bright, "Investigation of β-Cyclodextrin Immobilized at Silica Surfaces by Fluorescence Spectroscopy," Appl. Spectrosc. 46, 169-175 (1992)

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