Abstract

The development of a highly sensitive laser-induced fluorescence (LIF) detector for HPLC is described. It was specifically designed to monitor the fluorescence emitted from biogenic amines which were derivatized by naphthalenedicarboxaldehyde (NDA) in the presence of cyanide ion. The product of the amine derivatization, cyanobenz[f]isoindole (CBI), absorbs radiation at the output wavelength of the HeCd laser (441.6 nm). Optimization of detector sensitivity included evaluation of both optical components and cell configurations. A laser dye, coumarin 7, served as the test analyte because its native spectral properties coincided with those of the CBI-amine adducts and because no derivatization step was required for each injection. A limit of detection (LOD) on the order of 2 × 10⁻¹² M was reproducibly observed.

Laser-induced fluorescence detector with a fiber-coupled micro GRIN lens for capillary electrophoresis

Oluwatosin O. Dada
Appl. Opt. 59(16) 4849-4855 (2020)

Development of an ambient pressure laser-induced fluorescence instrument for nitrogen dioxide

Jeremy Parra and Linda A. George
Appl. Opt. 48(18) 3355-3361 (2009)

Development and initial calibration of a portable laser-induced fluorescence system used for in situ measurements of trace plastics and dissolved organic compounds in seawater and the Gulf of Mexico

Vasanthi Sivaprakasam, Robert F. Shannon, Caiyan Luo, Paula G. Coble, Jennifer R. Boehme, and Dennis K. Killinger
Appl. Opt. 42(33) 6747-6756 (2003)

Development of a photofragmentation laser-induced-fluorescence laser sensor for detection of 2,4,6-trinitrotoluene in soil and groundwater

Gary M. Boudreaux, Tracy S. Miller, Amanda J. Kunefke, Jagdish P. Singh, Fang-Yu Yueh, and David L. Monts
Appl. Opt. 38(9) 1411-1417 (1999)

Development of a field system for measurement of tropospheric OH radical using laser-induced fluorescence technique

Fengyang Wang, Renzhi Hu, Hao Chen, Pinhua Xie, Yihui Wang, Zhiyan Li, Huawei Jin, Jianguo Liu, and Wenqing Liu
Opt. Express 27(8) A419-A435 (2019)