Abstract

A completely optical method is described for the simultaneous determination of hemoglobin and myoglobin oxygen saturation. Solution oxygen concentrations were computed from measurements of phosphorescence decay of a soluble palladium porphyrin using the Stern-Volmer quenching relationship. Visible absorption spectra were recorded of hemoglobin/myoglobin mixtures progressively deoxygenated by bacterial aerobic metabolism. Separate hemoglobin and myoglobin oxygenation curves were resolved from the spectra of solutions containing both proteins by curve fitting, with the use of singular value decomposition of the spectra vs. oxygen concentration matrix. The method yielded a P₅₀ of 1.2 Torr for horse heart myoglobin at 24 ± 1°C, while the P₅₀ for sheep hemoglobin was 23 Torr at the same temperature. These values, obtained from mixtures, compare favorably with literature values determined for hemoglobin and myoglobin separately.

In vivo integrated photoacoustic and confocal microscopy of hemoglobin oxygen saturation and oxygen partial pressure

Yu Wang, Song Hu, Konstantin Maslov, Yu Zhang, Younan Xia, and Lihong V. Wang
Opt. Lett. 36(7) 1029-1031 (2011)

Spectral-profile-based algorithm for hemoglobin oxygen saturation determination from diffuse reflectance spectra

Po-Ching Chen and Wei-Chiang Lin
Biomed. Opt. Express 2(5) 1082-1096 (2011)

Quantitative monitoring of the level of singlet oxygen using luminescence spectra of phosphorescent photosensitizer

Peng Wang, Feng Qin, Zhiguo Zhang, and Wenwu Cao
Opt. Express 23(18) 22991-23003 (2015)

Oxygen distribution in the retinal and choroidal vessels of the cat as measured by a new phosphorescence imaging method

Ross D. Shonat, David F. Wilson, Charles E. Riva, and Marek Pawlowski
Appl. Opt. 31(19) 3711-3718 (1992)

Spectroscopic diffuse optical tomography for the quantitative assessment of hemoglobin concentration and oxygen saturation in breast tissue

Troy O. McBride, Brian W. Pogue, Ellen D. Gerety, Steven B. Poplack, Ulf L. Österberg, and Keith D. Paulsen
Appl. Opt. 38(25) 5480-5490 (1999)