Abstract

A fluorescence spectrometer has been constructed that simultaneously determines fluorescence intensity and excited-state lifetime. The ratio of intensity to lifetime is used to correct for the reduction in fluorescence intensity caused by collisional quenching. A frequency-domain technique is used to determine the excited-state lifetime in real-time. Computer-driven data acquisition provides instantaneous readout of the fluorescence intensity, the excited-state lifetime, and the ratio of these quantities. Correction for quenching by oxygen is demonstrated.

Correction of the quenching effect in two-dimensional laser-induced fluorescence measurement of laser-ablation processes

Yoshiki Nakata, Tatsuo Okada, and Mitsuo Maeda
Opt. Lett. 24(23) 1765-1767 (1999)

Quenching of neodymium fluorescence by molecular hydrogen

J. D. Prohaska, D. P. Machewirth, and E. Snitzer
Opt. Lett. 20(7) 719-721 (1995)

Fluorescence quenching by polystyrene microspheres in UV-visible and NIR tissue-simulating phantoms

Karthik Vishwanath, Wei Zhong, Melanie Close, and Mary-Ann Mycek
Opt. Express 14(17) 7776-7788 (2006)

Detection of oxygen by fluorescence quenching

M. E. Cox and B. Dunn
Appl. Opt. 24(14) 2114-2120 (1985)

Airborne laser-induced oceanic chlorophyll fluorescence: solar-induced quenching corrections by use of concurrent downwelling irradiance measurements

Frank E. Hoge, C. Wayne Wright, Robert N. Swift, and James K. Yungel
Appl. Opt. 37(15) 3222-3226 (1998)