Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 47,
  • Issue 10,
  • pp. 1594-1599
  • (1993)

Quantitative, Depth-Resolved Imaging of Oxygen Concentration by Phosphorescence Lifetime Measurement

Not Accessible

Your library or personal account may give you access

Abstract

Minimally invasive determination of oxygen concentration can be made by measuring phosphorescence lifetime. We describe a technique for depth-resolved measurements of oxygen concentration using confocal imaging of phosphorescence lifetime. A confocal imaging system is used to obtain depth-resolved measurements of phosphorescence decay. The spatial resolution of the system is characterized in terms of the Line Spread Function and shown to be similar in both lateral and depth directions. Lifetimes are calculated with the use of the Rapid Lifetime Determination technique. One- and two-dimensional quantitative images of oxygen concentration in a test specimen are rapidly generated from luminescence decay data. Measurements are made for oxygen concentrations ranging from 2 to 90 μM. A linear relationship between Pdmeso-tetra-(4-sulfonato-phenyl)-porphine phosphorescence inverse lifetime and oxygen concentration is determined. The presence of an intervening solution of different oxygen concentration does not significantly affect the concentration measurements made with the use of depth-resolved imaging techniques.

PDF Article
More Like This
Tomographic imaging of oxygen by phosphorescence lifetime

Sovia V. Apreleva, David F. Wilson, and Sergei A. Vinogradov
Appl. Opt. 45(33) 8547-8559 (2006)

Quantitation of cerebral oxygen tension using phasor analysis and phosphorescence lifetime imaging microscopy (PLIM)

Chang Liu, Amanda Chisholm, Buyin Fu, Clover T.-Y. Su, İkbal Şencan, Sava Sakadžić, and Mohammad A. Yaseen
Biomed. Opt. Express 12(7) 4192-4206 (2021)

Oxygen distribution and vascular injury in the mouse eye measured by phosphorescence-lifetime imaging

David F. Wilson, Sergei A. Vinogradov, Pavel Grosul, M. Noel Vaccarezza, Akiko Kuroki, and Jean Bennett
Appl. Opt. 44(25) 5239-5248 (2005)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved