Abstract

Quantitative analysis of peptides using internal standards was carried out with the use of matrix-assisted laser desorption/ionization (MALDI) on a time-of-flight mass spectrometer in a ferulic acid matrix. Spectra were collected and averaged over several areas of the sample surface at constant laser energy, and a defocused beam was used to improve reproducibility. A calibration curve was plotted, showing a correlation coefficient of 0.992 and a relative standard deviation of 1.83%. The accuracy and precision of this technique were sensitive to the crystal structure of the matrix and saturation effects of the instrument's detector.

Molecular analysis by ionization of laser-desorbed neutral species

Keith R. Lykke, Peter Wurz, Deborah H. Parker, and Michael J. Pellin
Appl. Opt. 32(6) 857-866 (1993)

Surface profiling of lithium with a laser-desorption mass-spectrometer microprobe

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Appl. Opt. 38(9) 1488-1493 (1999)

Resonance-ionization mass spectrometry of carbon

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J. Opt. Soc. Am. B 2(9) 1561-1565 (1985)

Chemical ionization Fourier transform mass spectrometry of chemical warfare agent simulants using laser-produced metal ions

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Appl. Opt. 29(33) 4981-4986 (1990)

Single-photon laser ionization time-of-flight mass spectroscopy detection in molecular-beam epitaxy: application to As₄, As₂, and Ga

Paul G. Strupp, April L. Alstrin, Russell V. Smilgys, and Stephen R. Leone
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