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  • Applied Spectroscopy
  • Vol. 48,
  • Issue 10,
  • pp. 1277-1281
  • (1994)

Laser Koehler Epi-Illumination for Raman and Fluorescence Microscopic Imaging

David M. Pallister and Michael D. Morris

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Abstract

A comparison of microscopic Raman images acquired with an optical-fiber critical (Nelson) illumination system, an optical-fiber Koehler laser illumination system, and Koehler laser illumination without an optical fiber demonstrates performance differences between the three illumination methods. Best images are obtained with optical-fiber Koehler illumination.

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