OSA's Digital Library

Applied Spectroscopy

Applied Spectroscopy


  • Vol. 48, Iss. 2 — Feb. 1, 1994
  • pp: 209–213

Influence of the Nitrobenzene Extraction Technique on the Protein Molecule Conformation

Ke Weizhong and Wu Jianzhong

Applied Spectroscopy, Vol. 48, Issue 2, pp. 209-213 (1994)

View Full Text Article

Acrobat PDF (476 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

  • Export Citation/Save Click for help


The Raman spectra of lysozyme, ribonuclease, and BSA (bovine serum albumin) in aqueous solution extracted by nitrobenzene and the ultra-violet visible absorption spectra of BSA in aqueous solution have been studied. It has been proven that the structure of all the characteristic Raman and UV bands of BAS protein in aqueous solution is due to α-helical and random-coil conformations. The tyrosine residue is "exposed", and the conformation of the carbon atoms in the disulfide bridge c-c-s-s-c-c is trans-gauche-gauche. The influence of adding nitrobenzene to lysozyme, ribonuclease, and BSA aqueous solution has been studied. The study indicated that nitrobenzene treatment of protein aqueous solution is an efficient means for obtaining better Raman spectra.

Ke Weizhong and Wu Jianzhong, "Influence of the Nitrobenzene Extraction Technique on the Protein Molecule Conformation," Appl. Spectrosc. 48, 209-213 (1994)

Sort:  Journal  |  Reset


References are not available for this paper.

Cited By

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited