OSA's Digital Library

Applied Spectroscopy

Applied Spectroscopy

| PUBLISHED BY SAS — AVAILABLE FROM SAS AND OSA

  • Vol. 48, Iss. 7 — Jul. 1, 1994
  • pp: 822–826

Resonance Raman Study of the Binding of the Anticancer Drug Amsacrine to DNA

Catherine A. Butler, Ralph P. Cooney, and William A. Denny

Applied Spectroscopy, Vol. 48, Issue 7, pp. 822-826 (1994)


View Full Text Article

Acrobat PDF (536 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations
  • Export Citation/Save Click for help

Abstract

The binding of amsacrine [4'-(9-acridinylamino)methanesulfon-m-anisidide] to calf thymus DNA was studied by UV-visible and resonance Raman spectroscopy. A shift of the UV-visible absorption band of amsacrine at 434 to 442 nm together with a decrease in the intensity of this band is observed upon amsacrine-DNA binding. The resonance Raman spectrum of DNA-bound amsacrine shows a general slight decrease in intensity relative to the spectrum of the free species. The significant decrease in intensity of the bands at 1165, 1265, and 1380 cm-1 upon binding to DNA is attributed to the formation of a single amsacrine-DNA species. The assignment of these bands (1165, 1265, and 1380 cm-1), which was based upon a previous normal coordinate analysis (NCA) and molecular neglect of diatomic overlap (MNDO) calculation, and the observed lack of shift in the band positions upon binding are consistent with intercalation being the major binding mode of amsacrine, as inferred previously by other techniques.

Citation
Catherine A. Butler, Ralph P. Cooney, and William A. Denny, "Resonance Raman Study of the Binding of the Anticancer Drug Amsacrine to DNA," Appl. Spectrosc. 48, 822-826 (1994)
http://www.opticsinfobase.org/as/abstract.cfm?URI=as-48-7-822

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited