Abstract

The detection and quantitation of the tetrols are important ways to study the extent of chemical damage to DNA. Both room- and low-temperature laser-excited solution fluorescence spectra of the four tetrol isomers were compared for their spectral features. The results showed that the characteristics of the solution spectra obtained at 77 K provided a very reliable means for distinguishing tetrol I-2 from its isomers. By utilizing both room-temperature solid-matrix (10% α-cyclodextrin/NaCl) fluorescence spectra from earlier work and solution florescence spectra at 77 K obtained in this work, it is possible to identify the four tetrol isomers.

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