OSA's Digital Library

Applied Spectroscopy

Applied Spectroscopy

| PUBLISHED BY SAS — AVAILABLE FROM SAS AND OSA

  • Vol. 50, Iss. 11 — Nov. 1, 1996
  • pp: 1345–1351

Spectrochemical Behavior of Carcinogenic Polynuclear Aromatic Hydrocarbons in Biological Systems. Part I: Steady-State Fluorometry of BaP and BeP in Living Cells

Ping Chiang, Kuang-Pang Li, and Tong-Ming Hseu

Applied Spectroscopy, Vol. 50, Issue 11, pp. 1345-1351 (1996)


View Full Text Article

Acrobat PDF (617 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations
  • Export Citation/Save Click for help

Abstract

The rates of interactions of polycyclic aromatic hydrocarbons (PAHs) with biological cells studied with fluorescence appear to be different for cells from different origins. The monomer emission of benzo[a]pyrene (BaP) is enhanced as in liposomes, but more significantly in normal liver cells than in liver cancer cells or kidney cells, and that enhancement is proportional to the amount of cells added. When PAHs are allowed to interact with cells for a certain period of time, metabolism appears to occur. The excimer emission is seen to dissipate continuously as reactions proceed, whereas the monomer emission increases, passes through a maximum, then starts to decrease when excimer emission becomes exhausted. The time plot of the BaP excimer emission in semilogarithmic coordinates indicates that the decrease or the mass transfer of microcrystalline BaP to the cell membrane is a first-order process. Metabolism has been investigated by monitoring the monomer emission. Liver cells have higher monomer emission than the kidney cells in the early stages of interaction, indicating that these cell membranes are more amenable to PAH absorption. Effects of vitamin K3 and radiation have also been investigated.

Citation
Ping Chiang, Kuang-Pang Li, and Tong-Ming Hseu, "Spectrochemical Behavior of Carcinogenic Polynuclear Aromatic Hydrocarbons in Biological Systems. Part I: Steady-State Fluorometry of BaP and BeP in Living Cells," Appl. Spectrosc. 50, 1345-1351 (1996)
http://www.opticsinfobase.org/as/abstract.cfm?URI=as-50-11-1345


Sort:  Journal  |  Reset

References

References are not available for this paper.

Cited By

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited