OSA's Digital Library

Applied Spectroscopy

Applied Spectroscopy

| PUBLISHED BY SAS — AVAILABLE FROM SAS AND OSA

  • Vol. 50, Iss. 2 — Feb. 1, 1996
  • pp: 148–153

Near-Infrared FT-SERS Microspectroscopy on Silver and Gold Surfaces: Technical Development, Mass Sensitivity, and Biological Applications

A. Beljebbar, G. D. Sockalingum, J. F. Angiboust, and M. Manfait

Applied Spectroscopy, Vol. 50, Issue 2, pp. 148-153 (1996)


View Full Text Article

Acrobat PDF (517 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations
  • Export Citation/Save Click for help

Abstract

An FT-Raman and FT-SERS microprobe with the spatial resolution on the micrometer scale has been developed. The main interfacing components are discussed and the whole setup is validated with the use of different SERS-active substrates: silver and gold colloids and gold island films. Micro-FT-SERS spectra of crocetin, mitoxantrone, and mitoxantrone/DNA complexes have been obtained, and the mass detection limits are found to be on the order of 5 × 102 molecules. Adsorption on the SERS-active substrates does not induce any detectable changes in the all-trans configuration of the crocetin. Adsorption of the mitoxantrone/DNA complex does not induce detectable perturbations of the molecular interactions within the complex. Moreover, interactions between the drug and DNA induces very similar effects in both the resonance Raman and FT-SERS spectra of the drugs. These effects were found to be consistent with the model of mitoxantrone/DNA intercalation proposed from nuclear magnetic resonance and resonance Raman data. The signal-to-noise ratios found indicate that submonolayer amounts of intracellularly localized drugs totaling less than 10-18 mole can be detected by means of the FT-SERS microprobe. Hence, both the extralow mass detection limit of the technique and its sensitivity to interactions within the supramolecular complexes will in the future allow the drug to be followed within the living cell.

Citation
A. Beljebbar, G. D. Sockalingum, J. F. Angiboust, and M. Manfait, "Near-Infrared FT-SERS Microspectroscopy on Silver and Gold Surfaces: Technical Development, Mass Sensitivity, and Biological Applications," Appl. Spectrosc. 50, 148-153 (1996)
http://www.opticsinfobase.org/as/abstract.cfm?URI=as-50-2-148


Sort:  Journal  |  Reset

References

References are not available for this paper.

Cited By

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited