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Applied Spectroscopy

Applied Spectroscopy

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  • Vol. 51, Iss. 11 — Nov. 1, 1997
  • pp: 1607–1612

Line-Scan Raman Microspectrometry for Biological Applications

C. J. De Grauw, C. Otto, and J. Greve

Applied Spectroscopy, Vol. 51, Issue 11, pp. 1607-1612 (1997)


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Abstract

A high-resolution confocal line-scan Raman microscope was developed for the study of biological samples such as cells and chromosomes. With the illumination of a line on the sample, all the spectral information of the line is recorded. The line-scan microscope was attained by the introduction of one scanning mirror, a slit, and two cylindrical lenses in a standard confocal Raman microscope. The resolution, for an ideal sample, is 0.5 mu m in the lateral direction and 3.5 mu m in the axial direction. Line-scan experiments were performed to demonstrate the applicability of the instrument for biological samples. Line-scan images of calcium phosphate bone implant coatings and of the banding pattern of polytene chromosomes are presented.

Citation
C. J. De Grauw, C. Otto, and J. Greve, "Line-Scan Raman Microspectrometry for Biological Applications," Appl. Spectrosc. 51, 1607-1612 (1997)
http://www.opticsinfobase.org/as/abstract.cfm?URI=as-51-11-1607


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