A high-resolution confocal line-scan Raman microscope was developed for the study of biological samples such as cells and chromosomes. With the illumination of a line on the sample, all the spectral information of the line is recorded. The line-scan microscope was attained by the introduction of one scanning mirror, a slit, and two cylindrical lenses in a standard confocal Raman microscope. The resolution, for an ideal sample, is 0.5 mu m in the lateral direction and 3.5 mu m in the axial direction. Line-scan experiments were performed to demonstrate the applicability of the instrument for biological samples. Line-scan images of calcium phosphate bone implant coatings and of the banding pattern of polytene chromosomes are presented.
C. J. De Grauw, C. Otto, and J. Greve, "Line-Scan Raman Microspectrometry for Biological Applications," Appl. Spectrosc. 51, 1607-1612 (1997)
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