OSA's Digital Library

Applied Spectroscopy

Applied Spectroscopy


  • Vol. 51, Iss. 11 — Nov. 1, 1997
  • pp: 1607–1612

Line-Scan Raman Microspectrometry for Biological Applications

C. J. De Grauw, C. Otto, and J. Greve

Applied Spectroscopy, Vol. 51, Issue 11, pp. 1607-1612 (1997)

View Full Text Article

Acrobat PDF (428 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

  • Export Citation/Save Click for help


A high-resolution confocal line-scan Raman microscope was developed for the study of biological samples such as cells and chromosomes. With the illumination of a line on the sample, all the spectral information of the line is recorded. The line-scan microscope was attained by the introduction of one scanning mirror, a slit, and two cylindrical lenses in a standard confocal Raman microscope. The resolution, for an ideal sample, is 0.5 mu m in the lateral direction and 3.5 mu m in the axial direction. Line-scan experiments were performed to demonstrate the applicability of the instrument for biological samples. Line-scan images of calcium phosphate bone implant coatings and of the banding pattern of polytene chromosomes are presented.

C. J. De Grauw, C. Otto, and J. Greve, "Line-Scan Raman Microspectrometry for Biological Applications," Appl. Spectrosc. 51, 1607-1612 (1997)

Sort:  Journal  |  Reset


References are not available for this paper.

Cited By

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited