A multivariate optimization approach, using a central composite blocked cube-star design, has been carried out to optimize the room-temperature phosphorescence (RTP) of a ternary complex between a polycyclic aromatic hydrocarbon (acenaphthene), beta -cyclodextrin, and two bromoalcohols (2-bromoethanol and 2,3-dibromopropanol). A comparative study of the effect of the two bromoalcohols in the phosphorescence response has shown that the highest RTP emission from acenaphthene included in beta -CD is obtained with 2,3-dibromopropanol. An RTP determination method for acenaphthene has been proposed. Calibration graphs range between 50 and 250 ng mL-1, and a detection limit of 24 ng mL-1 has been established.
A. Segura, Carretero C. Cruces, Blanco A. Fernandez, and Gutierrez A. Munoz De La Pena, "Optimization and Comparative Study of the RoomTemperature Phosphorescence of Acenaphthene in beta -Cyclodextrin in the Presence of Two Bromoalcohols," Appl. Spectrosc. 52, 420-425 (1998)