Abstract

The physical release of single granules from individual rat peritoneal mast cells (RPMCs) was monitored by video recording of the degranulating cells by a high-resolution charge-coupled device (CCD) microscope system. The bright granular core disappears from the image as the vesicular content is dissolved on contact with the extra-cellular fluid. After a fixed time delay, the exocytotic product, serotonin, was detected by capillary electrophoresis coupled with laser-induced native fluorescence (LINF-CE). The timing of the two events is mostly correlated, which supports a fast release mechanism of the granular products.

Terahertz-capillary electrophoresis (THz-CE) for direct detection of separated substances in solutions

Keiko Kitagishi, Takayuki Kawai, Masayoshi Tonouchi, and Kazunori Serita
Opt. Mater. Express 14(2) 472-482 (2024)

Laser-induced fluorescence detector with a fiber-coupled micro GRIN lens for capillary electrophoresis

Oluwatosin O. Dada
Appl. Opt. 59(16) 4849-4855 (2020)

Fluorescence monitoring of microchip capillary electrophoresis separation with monolithically integrated waveguides

Chaitanya Dongre, Ronald Dekker, Hugo J. W. M. Hoekstra, Markus Pollnau, Rebeca Martinez-Vazquez, Roberto Osellame, Giulio Cerullo, Roberta Ramponi, Rob van Weeghel, Geert A. J. Besselink, and Hans H. van den Vlekkert
Opt. Lett. 33(21) 2503-2505 (2008)

On-column refractive-index detection based on retroreflected beam interference for capillary electrophoresis

Yanzhuo Deng and Bincheng Li
Appl. Opt. 37(6) 998-1005 (1998)

Simultaneous scattering-absorption dual-modal cell imaging in a single shot by a transmission-mode photoacoustic microscope

Shiqing Wu, Chao Tao, Xiang Zhang, Feng Lu, and Xiaojun Liu
Opt. Lett. 45(20) 5832-5835 (2020)