Optical absorption and fluorescence spectral imaging is performed by using the recently developed fiber-bundle image compression (FIC) technique in which an entire spectral image is collected in a single scan of a charged-coupled device (CCD) detector. Absorption imaging is demonstrated by mapping the optical absorbance of a stained microscopic lily plant stem section. Fluorescence imaging is demonstrated by mapping shifts in the ruby R1 fluorescence line to determine the pressure distribution in a microcrystalline ruby powder squeezed between two diamond surfaces. The advantages and limitations of the FIC method, relative to tunable filter and line imaging techniques, are discussed.
Brian L. McClain, Jiaying, and Dor Ben-Amotz, "Optical Absorption and Fluorescence Spectral Imaging Using Fiber Bundle Image Compression," Appl. Spectrosc. 53, 1118-1122 (1999)
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