Abstract

In order to develop a high-throughput analysis method based upon surface-enhanced Raman scattering (SERS) spectroscopy, we have successfully tested the possibility of generating SERS-active silver surfaces from home-made AgX dispersions deposited in the wells of commercially available microtiter plates. In an effort to reduce the number of preparation steps, the SERS-active silver metal surface is generated from the silver halides <i>in situ</i> after sample application by the tightly focused Raman probe laser. The intensity of the SERS signal increases initially with the conditions for a high-enhancement factor becoming optimal. Later on, it decreases as the solvent is evaporated completely. The signal can, however, be restored to a great extent by adding new refreshing solvent, preferably methanol. Pilot experiments using aromatic thiols and amines as test analytes proved that sample volumes as small as 1 mL with analyte concentrations down to 10^-6 M are sufficient for recording of SERS spectra suitable to identify the analyte. A feasibility study was performed aimed at the identification of several analytes contained in the various fractions of the output of an analytical high-performance liquid chromatography (HPLC) instrument.

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