OSA's Digital Library

Applied Spectroscopy

Applied Spectroscopy

| PUBLISHED BY SAS — AVAILABLE FROM SAS AND OSA

  • Vol. 54, Iss. 3 — Mar. 1, 2000
  • pp: 384–389

Quantitative Detection of Alcian Blue 8GX in the Low Concentration Range Using Surface-Enhanced Resonance Raman Spectroscopy

Iqbal T. Shadi, Babur Z. Chowdhry, Martin J. Snowden, and Robert Withnall

Applied Spectroscopy, Vol. 54, Issue 3, pp. 384-389 (2000)


View Full Text Article

Acrobat PDF (281 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations
  • Export Citation/Save Click for help

Abstract

The viability of the application of surface-enhanced resonance Raman spectroscopy (SERRS) to the quantitative analysis of the dye alcian blue 8GX has been examined with the use of citrate- and borohydride-reduced silver colloids. A good linear correlation is observed for the dependence of the SERRS signal intensities at 1344 and 1536cm -1 (R=0.992 and 0.994, respectively) on dye concentrations in the range of 10 -9 -10 -7 M, when using the citrate-reduced silver sol. Likewise, a good linear correlation is also observed for the dependence of the SERRS signal intensities at 1344 and 1536cm -1 (R=0.978 and 0.959, respectively) on dye concentrations in the range 10 -10 -10 -9 M, when using the borohydride-reduced silver sol. At concentrations of dye above 10 -7 M, the concentration dependence of the SERRS signals is nonlinear, almost certainly due to the coverage of the surface of the colloidal silver particles by the dye being in excess of a full monolayer. However, at concentrations of alcian blue greater than 10 -7 M, resonance Raman spectroscopy (RRS) can be employed for quantitative analysis of the dye. When performing RRS, using methanol as an internal standard, we observed a good linear correlation for the dependence of the signal intensities at 1344 and 1536cm -1 (R=0.995 for both bands) on the dye concentration in the 10 -6 -10 -3 M range. The limits of detection of alcian blue by SERRS using a citrate-reduced silver sol, SERRS using a borohydride-reduced silver sol, RRS, and visible absorption spectroscopy are found to be 26 ppb, 203 ppt, 2 ppm, and 0.7 ppm, respectively. Index Headings: Alcian blue; Copper phthalocyanine; Silver sol; Surface-enhanced resonance Raman spectroscopy; SERRS; Resonance Raman spectroscopy; RRS; Quantitative analysis; Internal standard.

Citation
Iqbal T. Shadi, Babur Z. Chowdhry, Martin J. Snowden, and Robert Withnall, "Quantitative Detection of Alcian Blue 8GX in the Low Concentration Range Using Surface-Enhanced Resonance Raman Spectroscopy," Appl. Spectrosc. 54, 384-389 (2000)
http://www.opticsinfobase.org/as/abstract.cfm?URI=as-54-3-384

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited