Abstract
It has been shown that fluorescence spectroscopy of sugar in aqueous solution carries important quality and process information related to beet sugar factories, which is accessible by multivariate analyses. A method for measuring crystalline sugar directly on-line in the process should be advantageous. In this paper we compare the solution measurement technique with two methods of fluorescence measurement on solid sugar. Surprisingly, it was possible to measure fluorescence through the sugar crystals by using the same transmission techniques with 90 detection as with the sugar solutions. This method was compared with a 45 front-surface reflection method. Sugar samples from six different sugar factories were examined. The spectral responses were reasonable, but they were influenced by the heterogeneous sample composition and the sample geometry. It was possible with the two methods to separate sugar samples according to factory with the use of principal component analysis (PCA). Seasonal time trends were found in weekly samples from the same factory. Partial least-squares regression (PLS) was used to predict quality parameters, where color (range: 6-41), ash (range: 0.003-0.018), and alpha -amino-N (range: 0.28-5.07) could be modeled with errors of 2.3-2.6, 0.0015-0.0016, and 0.40-0.42, respectively. Model errors for similar solution data have been determined to 2.4, 0.0012, and 0.266, respectively. Index Headings: Fluorescence; Sugar; Solid sample; Chemometrics; Multivariate calibration; Principal component analysis; PCA; Partial least-squares; PLS.
PDF Article
More Like This
Multiple kinds of pesticide residue detection using fluorescence spectroscopy combined with partial least-squares models
Rendong Ji, Shicai Ma, Hua Yao, Yue Han, Xiao Yang, Ruiqiang Chen, Yinshang Yu, Xiaoyan Wang, Dongyang Zhang, TieZhu Zhu, and Haiyi Bian
Appl. Opt. 59(6) 1524-1528 (2020)
Cited By
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Login to access Optica Member Subscription