OSA's Digital Library

Applied Spectroscopy

Applied Spectroscopy

| PUBLISHED BY SAS — AVAILABLE FROM SAS AND OSA

  • Vol. 55, Iss. 11 — Nov. 1, 2001
  • pp: 1495–1501

Method for Measuring Cellular Optical Absorption and Scattering Evaluated Using Dilute Cell Suspension Phantoms

A. Sefkow, M. Bree, and M.-A. Mycek

Applied Spectroscopy, Vol. 55, Issue 11, pp. 1495-1501 (2001)


View Full Text Article

Acrobat PDF (235 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations
  • Export Citation/Save Click for help

Abstract

The opal glass method (OGM) is a standard technique used by the ocean optics community to measure the spectral absorption coefficients of algal suspensions. We present and evaluate this method for its potential application to measurements of the inherent optical properties (optical absorption and scattering coefficients) of dilute mammalian cell suspensions, as an alternative to the integrating sphere method. In a series of controlled experiments on absorbing and scattering phantoms (Evans blue dye and polystyrene spheres 1-20 μm in diameter), separation of absorption and scattering was possible with less than 20% error in absorption coefficient estimates for particles with major scattering components in the size range of 2-5 μm.

Citation
A. Sefkow, M. Bree, and M.-A. Mycek, "Method for Measuring Cellular Optical Absorption and Scattering Evaluated Using Dilute Cell Suspension Phantoms," Appl. Spectrosc. 55, 1495-1501 (2001)
http://www.opticsinfobase.org/as/abstract.cfm?URI=as-55-11-1495

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

If you are accessing the full text through a member bundle, please use the Enhanced HTML link to gain access to the citation lists and other restricted features. Note that accessing both the PDF and HTML versions of an article will count as only one download against your account.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

If you are accessing the full text through a member bundle, please use the Enhanced HTML link to gain access to the citation lists and other restricted features. Note that accessing both the PDF and HTML versions of an article will count as only one download against your account.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited