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Applied Spectroscopy

Applied Spectroscopy

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  • Vol. 56, Iss. 11 — Nov. 1, 2002
  • pp: 1484–1489

FT-NIR Spectroscopic Analysis of Nitrogen in Cotton Leaves

Mark R. Riley and Loreto C. Cánaves

Applied Spectroscopy, Vol. 56, Issue 11, pp. 1484-1489 (2002)


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Abstract

Near-infrared spectroscopy was evaluated as a means to quantify the nitrogen content in fresh cotton leaves (Gossypium hirsutum L. var. Delta Pine 90) subjected to a factorial design experiment of varying nitrogen and water applications. Absorbance spectra were collected in the 10 000-4000 cm-1 (1000-2500 nm) region from fresh cotton leaves over a two month portion of the growing season. Total nitrogen content was quantified by a wet chemistry Kjeldahl method for validation purposes. Partial least-squares regression analysis, using an automated grid search method, selected the spectral region 6041 to 5651 cm-1 (1650-1770 nm) for analysis based on having the lowest standard error of prediction of total nitrogen content. This region includes protein spectral features. Nitrogen predictions resulted in a correlation coefficient of 0.83, and a standard error of prediction of 0.29% for nitrogen levels ranging from 3.1 to 5.2% total nitrogen. This approach has promise for providing rapid plant chemical analyses for cotton crop fertilization management purposes.

Citation
Mark R. Riley and Loreto C. Cánaves, "FT-NIR Spectroscopic Analysis of Nitrogen in Cotton Leaves," Appl. Spectrosc. 56, 1484-1489 (2002)
http://www.opticsinfobase.org/as/abstract.cfm?URI=as-56-11-1484

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