Abstract

A method is described for the construction of three-dimensional infrared images from serial two-dimensional infrared images of various chemical structure parameters acquired from cortical bone. A series of serial cortical bone sections, each 4-5 μm thick, separated by ~100 μm, were microtomed from PMMA-embedded normal human tibia. Twenty-three IR image cubes were acquired from this series, corresponding to an overall sample length of ~2.2 mm. From each image cube, IR images of the spatial distribution of the mineral-to-matrix (protein) ratio, the mineral crystallinity, and the ratio of nonreducible-to-reducible collagen crosslinks were calculated. Two-dimensional images were stacked to form a 3D view of the cortical bone, with contrast provided by variation in the spatial distribution of any of the aforementioned chemical/molecular structural parameters.

Three-dimensional reconstruction of a bone image from cone beam projections

Nagaaki Ohyama, Soichi Inoue, Hideaki Haneishi, Jumpei Tsujiuchi, and Toshio Honda
Appl. Opt. 28(24) 5338-5341 (1989)

Spatially offset Raman spectroscopy for in vivo bone strength prediction

Chi Shu, Keren Chen, Maria Lynch, Jason R. Maher, Hani A. Awad, and Andrew J. Berger
Biomed. Opt. Express 9(10) 4781-4791 (2018)

Three-dimensional terahertz computed tomography of human bones

Maryelle Bessou, Bruno Chassagne, Jean-Pascal Caumes, Christophe Pradère, Philippe Maire, Marc Tondusson, and Emmanuel Abraham
Appl. Opt. 51(28) 6738-6744 (2012)

Exploiting FTIR microspectroscopy and chemometric analysis in the discrimination between Egyptian ancient bones: a case study

A. El-Hussein, I. Yousef, and M. A. Kasem
J. Opt. Soc. Am. B 37(11) A110-A120 (2020)

Surface structural damage study in cortical bone due to medical drilling

Cesar G. Tavera R., Manuel H. De la Torre-I, Jorge M. Flores-M., Ma Del Socorro Hernandez M., Fernando Mendoza-Santoyo, Manuel de J. Briones-R., and Jorge Sanchez-P.
Appl. Opt. 56(13) F179-F188 (2017)