Abstract
We determine the pH dependency of the mid-infrared spectra in aqueous solution of the organic dissociative materials in the metabolic pathway: saccharide phosphates (G6P, F6P), adenosine, and its phosphates (ATP, ADP, AMP). The series of molar absorbance spectra for these reagents were obtained in a pH range of about 2 to 11 with a Fourier transform infrared (FT-IR) spectrometer equipped with a horizontal diamond attenuated total reflection (ATR) sampling accessory. We also provide a method of infrared spectral extraction of ionic dissociative materials by performing a linear least-square fitting utilizing the formulas of ionic dissociation equilibrium shift, and we obtain the infrared spectrum of each ionic species of the dissociative materials: G6P<sup>-</sup>, G6P<sup>2-</sup>; F6P<sup>-</sup>, F6P<sup>2-</sup>; ATP<sup>2-</sup>, ATP<sup>3-</sup>, ATP<sup>4-</sup>; ADP<sup>-</sup>, ADP<sup>2-</sup>, ADP<sup>3-</sup>; AMP, AMP<sup>-</sup>, AMP<sup>2-</sup>; and adenosine<sup>+</sup>, adenosine<sup>0</sup>. The infrared spectral structure of each ionic species of the dissociative materials in the metabolic pathway are discussed. Additionally, the possibility for a quantification system of the concentrations of the organic dissociative materials in varying pH is suggested.
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