Abstract

Confocal Raman microscopy data are reported for a laminated polymer (Paramount) and for pigskin. The nature of the laminated structure of the polymer provides a useful test for evaluation of thickness distortions in confocal measurements in soft samples, which are found to be quite significant. The spatial variation in line profiles generated from univariate analyses with scores derived from factor loadings are consistent for both samples and provide distinct diagnostic markers for stratum corneum and epidermis regions of skin. Univariate analysis of the C-C stretching region of skin reveals a spatial dependence of chain conformational order. In addition, variations in keratin-containing areas of the stratum corneum are readily identified from area maps of the S-S stretching vibrations. These data indicate that confocal Raman imaging studies of molecular structure changes in particular regions of skin during pathological processes will prove quite valuable in dermatology.

Characterizing stratum corneum structure, barrier function, and chemical content of human skin with coherent Raman scattering imaging

Sam Osseiran, Jomer Dela Cruz, Sinyoung Jeong, Hequn Wang, Christina Fthenakis, and Conor L. Evans
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Biophysical basis of skin cancer margin assessment using Raman spectroscopy

Xu Feng, Matthew C. Fox, Jason S. Reichenberg, Fabiana C. P. S. Lopes, Katherine R. Sebastian, Mia K. Markey, and James W. Tunnell
Biomed. Opt. Express 10(1) 104-118 (2019)

Raman active components of skin cancer

Xu Feng, Austin J Moy, Hieu T. M. Nguyen, Jason Zhang, Matthew C. Fox, Katherine R. Sebastian, Jason S. Reichenberg, Mia K. Markey, and James W. Tunnell
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Frequency-dependent skin penetration depth of terahertz radiation determined by water sorption–desorption

Borwen You, Ching-Yu Chen, Chin-Ping Yu, Pei-Hwa Wang, and Ja-Yu Lu
Opt. Express 26(18) 22709-22721 (2018)

Non-invasive depth profiling of the stratum corneum in vivo using confocal Raman microscopy considering the non-homogeneous distribution of keratin

Maxim E. Darvin, Chun-Sik Choe, Johannes Schleusener, and Jürgen Lademann
Biomed. Opt. Express 10(6) 3092-3103 (2019)