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Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 60,
  • Issue 4,
  • pp. 347-351
  • (2006)

Temperature-Jump Apparatus with Raman Detection Based on a Solid-State Tunable (1.80–2.05 μm) kHz Optical Parametric Oscillator Laser

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Abstract

The operating characteristics of a pulsed (10 ns) tunable near-infrared (NIR) laser source are described for temperature-jump (T-jump) applications. A Q-switched Nd:YLF laser (∼10 ns pulses) with a 1 kHz repetition rate is used to pump a potassium titanyl arsenate (KTA) crystal-based optical parametric oscillator (OPO), producing ∼1 mJ NIR pulses that are tunable (1.80–2.05 μm) across the 1.9 μm vibrational overtone band of water. This T-jump source has been coupled to a deep ultraviolet (UV) probe laser for Raman studies of protein dynamics. T-jumps of up to 30 °C, as measured via the O–H stretching Raman band of water, are readily achieved. Application to cytochrome c unfolding is demonstrated.

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