Abstract

Raman spectroscopy has long been used for the chemical analysis of organic matter, including natural products, using excitation wavelengths in the visible, infrared, or ultraviolet portions of the spectrum. The use of ultraviolet resonance Raman spectroscopy (UVRR) to study bulk samples of plant tissue has typically been carried out by rotating homogeneous macro-samples beneath the laser beam in order to minimize the amount of UV radiation impinging on any one spot, thereby avoiding its potentially damaging effects on the organic matter analyzed. This paper extends the use of UVRR to the study on a microscopic scale of individual plant cell walls by use of the controlled micro-displacement of a sample.

Refractive Index of Plant Cell Walls

H. W. Gausman, W. A. Allen, and D. E. Escobar
Appl. Opt. 13(1) 109-111 (1974)

Fiber-optic probes with improved excitation and collection efficiency for deep-UV Raman and resonance Raman spectroscopy

L. Shane Greek, H. Georg Schulze, Michael W. Blades, Charles A. Haynes, Karl-Friedrich Klein, and Robin F. B. Turner
Appl. Opt. 37(1) 170-180 (1998)

Absorption coefficient and purine photobleaching rate in colon mucosa during resonance Raman spectroscopy at 251 nm

Nada N. Boustany
Appl. Opt. 40(34) 6396-6405 (2001)

Rational design of fiber-optic probes for visible and pulsed-ultraviolet resonance Raman spectroscopy

L. Shane Greek, H. Georg Schulze, Charles A. Haynes, Michael W. Blades, and Robin F. B. Turner
Appl. Opt. 35(21) 4086-4095 (1996)

Development of a time-gated system for Raman spectroscopy of biological samples

Florian Knorr, Zachary J. Smith, and Sebastian Wachsmann-Hogiu
Opt. Express 18(19) 20049-20058 (2010)