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Applied Spectroscopy

Applied Spectroscopy

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  • Vol. 60, Iss. 9 — Sep. 1, 2006
  • pp: 994–1003

Surface Plasmon Resonance Investigations of Human Epidermal Growth Factor Receptor 2

V. S. Martin, B. A. Sullivan, K. Walker, H. Hawk, B. P. Sullivan, and L. J. Noe

Applied Spectroscopy, Vol. 60, Issue 9, pp. 994-1003 (2006)


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Abstract

This investigation utilizes surface plasmon resonance (SPR) spectroscopy to detect and quantify human epidermal growth factor receptor 2 (HER-2), an oncogene product that is over-expressed in some aggressive forms of breast cancer. Specifically, the HER-2 trans-membrane protein p185 and its extra cellular fragment p105 are analytes targeted in this work by using a gold-based biosensor slide on which an anti-HER-2 antibody has been immobilized by attachment to Protein G that is fixed to the gold film. A detection limit of ≥11 ng/mL for p185 resulted when trastuzumab was used as the anti-HER-2 antibody on the biosensor slide. Experiments with semi-purified p105 revealed that it binds weakly and reversibly to trastuzumab, therefore complicating its detection and quantification. Results of studies that reacted a 13-amino-acid peptide (PP13) from the HER-2 kinase domain with its specific antibody were critically different than p185 and p105 studies. Spectral analysis of the reflectivity at constant bulk buffer refractive index revealed a progressive negative SPR shift over time. A negative shift suggests that a loss of protein mass from the anti-PP13 antibody–Protein G biosensor is occurring. Several possibilities that may explain these negative SPR shifts are discussed.

Citation
V. S. Martin, B. A. Sullivan, K. Walker, H. Hawk, B. P. Sullivan, and L. J. Noe, "Surface Plasmon Resonance Investigations of Human Epidermal Growth Factor Receptor 2," Appl. Spectrosc. 60, 994-1003 (2006)
http://www.opticsinfobase.org/as/abstract.cfm?URI=as-60-9-994


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