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Applied Spectroscopy

Applied Spectroscopy


  • Vol. 61, Iss. 11 — Nov. 1, 2007
  • pp: 1233–1237

Raman Spectroscopic Measurement of Relative Concentrations in Mixtures of Oral Bacteria

Qingyuan Zhu, Robert G. Quivey, and Andrew J. Berger

Applied Spectroscopy, Vol. 61, Issue 11, pp. 1233-1237 (2007)

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Near-infrared Raman spectroscopy has been used for species identification of pure microbial specimens for more than a decade. More recently, this optical method has been extended to the analysis of specimens containing multiple species. In this report, we demonstrate rapid, reagent-free quantitative analysis of a simplified model of oral plaque containing three oral bacteria species, S. mutans, S. sanguis, and S. gordonii, using near-infrared Raman spectroscopy. Raman spectra were acquired from bacterial mixtures in 200 seconds. A prediction model was calibrated by the partial least squares method and validated by additional samples. On a scale from 0 to 1, relative fractions of each species could be predicted with a root mean square error of 0.07. These results suggest that near-infrared Raman spectroscopy is potentially useful in quantification of microbial mixtures in general and oral plaques in particular.

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Qingyuan Zhu, Robert G. Quivey, and Andrew J. Berger, "Raman Spectroscopic Measurement of Relative Concentrations in Mixtures of Oral Bacteria," Appl. Spectrosc. 61, 1233-1237 (2007)

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