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Applied Spectroscopy

Applied Spectroscopy

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  • Vol. 61, Iss. 11 — Nov. 1, 2007
  • pp: 1233–1237

Raman Spectroscopic Measurement of Relative Concentrations in Mixtures of Oral Bacteria

Qingyuan Zhu, Robert G. Quivey, and Andrew J. Berger

Applied Spectroscopy, Vol. 61, Issue 11, pp. 1233-1237 (2007)


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Abstract

Near-infrared Raman spectroscopy has been used for species identification of pure microbial specimens for more than a decade. More recently, this optical method has been extended to the analysis of specimens containing multiple species. In this report, we demonstrate rapid, reagent-free quantitative analysis of a simplified model of oral plaque containing three oral bacteria species, <i>S. mutans</i>, <i>S. sanguis</i>, and <i>S. gordonii</i>, using near-infrared Raman spectroscopy. Raman spectra were acquired from bacterial mixtures in 200 seconds. A prediction model was calibrated by the partial least squares method and validated by additional samples. On a scale from 0 to 1, relative fractions of each species could be predicted with a root mean square error of 0.07. These results suggest that near-infrared Raman spectroscopy is potentially useful in quantification of microbial mixtures in general and oral plaques in particular.

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Vol. 2, Iss. 12 Virtual Journal for Biomedical Optics

Citation
Qingyuan Zhu, Robert G. Quivey, and Andrew J. Berger, "Raman Spectroscopic Measurement of Relative Concentrations in Mixtures of Oral Bacteria," Appl. Spectrosc. 61, 1233-1237 (2007)
http://www.opticsinfobase.org/as/abstract.cfm?URI=as-61-11-1233


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