OSA's Digital Library

Applied Spectroscopy

Applied Spectroscopy

| PUBLISHED BY SAS — AVAILABLE FROM SAS AND OSA

  • Vol. 62, Iss. 6 — Jun. 1, 2008
  • pp: 689–700

Method for the Estimation of the Mean Lorentzian Bandwidth in Spectra Composed of an Unknown Number of Highly Overlapped Bands

Víctor A. Lóenz-Fonfría and Esteve Padrós

Applied Spectroscopy, Vol. 62, Issue 6, pp. 689-700 (2008)


View Full Text Article

Acrobat PDF (576 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations
  • Export Citation/Save Click for help

Abstract

We introduce a method for the estimation of the mean Lorentzian bandwidth of the component bands in a spectrum. The method is computationally simple, using only the module of the Fourier transform of the spectrum, and its first derivative. Moreover, the presented method does not require knowledge of the number of bands in the spectrum, their band positions, or their band areas. Furthermore, it works on spectra containing Lorentzian bands, as well as Gaussian and Voigtian bands. Therefore, the introduced method seems especially well suited for obtaining a representative Lorentzian width for highly overlapped bands, independent of their number and Lorentzian/Gaussian character. We describe how different experimental limitations (spectral truncation, offset error, presence of noise, etc.) may affect the performance of the method, and when required we propose effective alternatives to minimize their effects. Finally, we show the application of the method to an experimental spectrum: the amide I band of a dry film of the solubilized ADP/ATP carrier. The estimation of the mean Lorentzian width can allow, for instance, for a more objective selection of the deconvolution width in Fourier self-deconvolution, allowing for a more objective and reliable analysis of the amide I band of proteins. The mean Lorentzian width can also be useful to obtain an estimation of the homogenous broadening and vibrational relaxation of the amide I vibration of proteins, without requiring complex pump-probe experiments.

Virtual Issues
Vol. 3, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Víctor A. Lóenz-Fonfría and Esteve Padrós, "Method for the Estimation of the Mean Lorentzian Bandwidth in Spectra Composed of an Unknown Number of Highly Overlapped Bands," Appl. Spectrosc. 62, 689-700 (2008)
http://www.opticsinfobase.org/as/abstract.cfm?URI=as-62-6-689

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited