OSA's Digital Library

Applied Spectroscopy

Applied Spectroscopy

| PUBLISHED BY SAS — AVAILABLE FROM SAS AND OSA

  • Vol. 63, Iss. 1 — Jan. 1, 2009
  • pp: 14–24

Classification of Select Category A and B Bacteria by Fourier Transform Infrared Spectroscopy

Alan C. Samuels, A. Peter Snyder, Darren K. Emge, Diane ST. Amant, Jennifer Minter, Mark Campbell, and Ashish Tripathi

Applied Spectroscopy, Vol. 63, Issue 1, pp. 14-24 (2009)


View Full Text Article

Acrobat PDF (1393 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations
  • Export Citation/Save Click for help

Abstract

Fourier transform infrared (FT-IR) spectroscopy historically is a powerful tool for the taxonomic classification of bacteria by genus, species, and strain when they are grown under carefully controlled conditions. Relatively few reports have investigated the determination and classification of pathogens such as the National Institute of Allergy and Infectious Diseases (NIAID) Category A Bacillus anthracis spores and cells (BA), Yersinia species, Francisella tularensis (FT), and Category B Brucella species from FT-IR spectra. We investigated the multivariate statistics classification ability of the FT-IR spectra of viable pathogenic and non-pathogenic NIAID Category A and B bacteria. The impact of different growth media, growth time and temperature, rolling circle filter of the data, and wavelength range were investigated for their microorganism differentiation capability. Viability of the bacteria was confirmed by agar plate growth after the FT-IR experimental procedures were performed. Principal component analysis (PCA) was reduced to maps of two PC vectors in order to distill the FT-IR spectral features into manageable, visual presentations. The PCA results of the strains of BA, FT, Brucella, and Yersinia spectra from conditions of varying growth media and culture time were readily separable in two-dimensional (2D) PC plots. FT spectra were separated from those of the three other genera. The BA pathogenic spore strains 1029, LA1, and Ames were clearly differentiated from the rest of the dataset. Yersinia rhodei, Y. enterocolitica, and Y. pestis species were distinctly separated from the remaining dataset and could also be classified by growth media. Different growth media produced distinct subsets in the FT, BA, and Yersinia spp. regions in the 2D PC plots. Various 2D PC plots provided differential degrees of separation with respect to the four viable bacterial genera including the BA sub-categories of pathogenic spores, vegetative cells, and nonpathogenic vegetative cells. This work provided evidence that FT-IR spectroscopy can indeed separate the four major pathogenic bacterial genera of NIAID Category A and B biological threat agents including details according to the growth conditions and statistical parameters.

Virtual Issues
Vol. 4, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Alan C. Samuels, A. Peter Snyder, Darren K. Emge, Diane ST. Amant, Jennifer Minter, Mark Campbell, and Ashish Tripathi, "Classification of Select Category A and B Bacteria by Fourier Transform Infrared Spectroscopy," Appl. Spectrosc. 63, 14-24 (2009)
http://www.opticsinfobase.org/as/abstract.cfm?URI=as-63-1-14

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited