Near-infrared Raman spectroscopy is a powerful analytical tool for detecting critical differences in biological samples with minimum interference in the Raman spectra from the native fluorescence of the samples. The technique is often suggested as a potential screening tool for cancer. In this article we report in vitro Raman spectra of squamous cells in normal and cancerous cervical human tissue from seven patients, which have good signal-to-noise ratio and which were found to be reproducible. These preliminary results show that several Raman features in these spectra could be used to distinguish cancerous cervical squamous cells from normal cervical squamous cells. In general, the Raman spectra of cervical cancer cells show intensity differences compared to those of normal squamous cell spectra. For example, several well-defined Raman peaks of collagen in the 775 to 975 cm−1 region are observed in the case of normal squamous cells, but these are below the detection limit of normal Raman spectroscopy in the spectra of invasive cervical cancer cells. In the high frequency 2800 to 3100 cm−1 region, it is found that the peak area under the CH stretching band is lower by a factor of approximately six in the spectra of cervical cancer cells as compared with that of the normal cells. The Raman chemical maps of regions of cancer and normal cells in the cervical epithelium made from the spectral features in the 775 to 975 cm−1 and 2800 to 3100 cm−1 regions are also found to show good correlation with each other.
Vol. 5, Iss. 6 Virtual Journal for Biomedical Optics
Lori E. Kamemoto, Anupam K. Misra, Shiv K. Sharma, Marc T. Goodman, Hugh Luk, Ava C. Dykes, and Tayro Acosta, "Near-Infrared Micro-Raman Spectroscopy for in Vitro Detection of Cervical Cancer," Appl. Spectrosc. 64, 255-261 (2010)