OSA's Digital Library

Applied Spectroscopy

Applied Spectroscopy

| PUBLISHED BY SAS — AVAILABLE FROM SAS AND OSA

  • Vol. 64, Iss. 3 — Mar. 1, 2010
  • pp: 324–327

Investigation of Saturation and Photobleaching of Allophycocyanin by Single-Molecule Recrossing Events

Yu Tian and Dimitri Pappas

Applied Spectroscopy, Vol. 64, Issue 3, pp. 324-327 (2010)


View Full Text Article

Acrobat PDF (723 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations
  • Export Citation/Save Click for help

Abstract

Phycobiliprotein fluorescent labels are playing an increasingly important role in bioanalysis. They are also being used more and more frequently as light-harvesting materials for energy research. It is therefore critical to study the working conditions of these fluorescent dyes. Allophycocyanin (APC) belongs to a group of phycobiliproteins and features red excitation and emission, making it both a useful fluorophore and light-harvesting material. Saturation irradiance and photobleaching of APC were studied by single-molecule detection in this work. The mean fluorescence intensity at different laser powers was calculated from extracted single-molecule fluorescence peaks. By interpolating the figure of the mean fluorescence intensity as a function of excitation power, the experimental saturation irradiance can be extracted. By comparing the experimental with the calculated saturation irradiance, it can be demonstrated that the triplet state for APC was formed at higher excitation irradiance. The technique of molecular recrossing events was applied to investigate the photobleaching of APC. Normalized recrossing events confirmed that photobleaching occurred at high excitation power. This work provided the optimizing experimental conditions for APC both as a fluorophore and as a light-harvesting molecule.

Virtual Issues
Vol. 5, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Yu Tian and Dimitri Pappas, "Investigation of Saturation and Photobleaching of Allophycocyanin by Single-Molecule Recrossing Events," Appl. Spectrosc. 64, 324-327 (2010)
http://www.opticsinfobase.org/as/abstract.cfm?URI=as-64-3-324

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited