OSA's Digital Library

Applied Spectroscopy

Applied Spectroscopy

| PUBLISHED BY SAS — AVAILABLE FROM SAS AND OSA

  • Vol. 64, Iss. 8 — Aug. 1, 2010
  • pp: 901–906

Fourier Transform Infrared Microspectroscopy of Endocarditis Vegetation

Eric Batard, Frédéric Jamme, David Boutoille, Cédric Jacqueline, Jocelyne Caillon, Gilles Potel, and Paul Dumas

Applied Spectroscopy, Vol. 64, Issue 8, pp. 901-906 (2010)


View Full Text Article

Acrobat PDF (3305 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations
  • Export Citation/Save Click for help

Abstract

The objectives of this work were to compare the infrared spectra of bacterial endocarditis vegetation with those of native valvular tissue and the infrared spectra of vegetation bacterial masses with those of surrounding vegetation tissue. Streptococcal aortic endocarditis was induced in three rabbits. Vegetation slices were cryo-sectioned for study by Fourier transform infrared (FT-IR) microspectroscopy. Valvular apparatus, vegetation, and bacterial masses within the vegetation were localized on hematoxylin and eosin (H&E) stained contiguous slices. Infrared images of whole vegetations and images of bacterial masses were acquired with apertures set to 80 × 80 and 20 × 20 μm, respectively. Valvular apparatus and vegetation showed different infrared spectra, mainly in the amide I and amide II bands (1674–1518 cm−1), and at about 1450, 1400, 1340, 1280, 1240, 1200, 1080, and 1030 cm−1. Valvular collagen, elastin, and proteoglycans may explain these differences. Bacterial masses and surrounding vegetation showed different infrared patterns, mainly in the amide I and amide II bands and in the 1142–991 cm−1 carbohydrate spectral range. Bacterial nucleic acids and polysaccharides may partly explain these differences. Study of experimental endocarditis vegetation using FT-IR microspectroscopy distinguishes (1) the vegetation from the valvular tissue, and (2) the bacterial masses from the surrounding tissue. This study demonstrates for the first time that FT-IR microspectroscopy is able to detect bacterial growth in infected tissue. FT-IR microspectroscopy appears to be a useful tool for investigation of the biochemical structure of endocarditis vegetation.

Virtual Issues
Vol. 5, Iss. 12 Virtual Journal for Biomedical Optics

Citation
Eric Batard, Frédéric Jamme, David Boutoille, Cédric Jacqueline, Jocelyne Caillon, Gilles Potel, and Paul Dumas, "Fourier Transform Infrared Microspectroscopy of Endocarditis Vegetation," Appl. Spectrosc. 64, 901-906 (2010)
http://www.opticsinfobase.org/as/abstract.cfm?URI=as-64-8-901


Sort:  Journal  |  Reset

References

References are not available for this paper.

Cited By

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited