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Applied Spectroscopy

Applied Spectroscopy

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  • Vol. 64, Iss. 8 — Aug. 1, 2010
  • pp: 942–948

Heterogeneous Thermal-Lens Immunoassay for Small Organic Compounds: Determination of 4-Aminophenol

Dmitry A. Nedosekin, Anton V. Brusnichkin, Andrei Y. Luk'yanov, Sergei A. Eremin, and Mikhail A. Proskurnin

Applied Spectroscopy, Vol. 64, Issue 8, pp. 942-948 (2010)


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Abstract

4-Aminophenol was selected as a model hapten for thermal-lens detection in a heterogeneous indirect immunoassay for small organic compounds. The assay is based on the competition of the free hapten and 4-aminophenol labeled with tetramethyl rhodamine isothiocyanate (TRITC) for rabbit anti-4-aminophenol antibodies (4-AP-GA-BSA) immobilized on a transparent poly(ethylene terephthalate) plate. The amount of the colored analyte was directly measured by thermal lensing at the plate surface. The developed method is specific for 4-aminophenol. The limit of detection is 2 × 10−7 M, which corresponds to the absolute amount of 3 × 10−11 mol of 4-aminophenol. The sensitivities of the developed thermal-lens and fluorescence-based measurements of the immunoassay under the same conditions are in good agreement with the expected and discussed theoretical sensitivities of photothermal and fluorescence measurements.

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Citation
Dmitry A. Nedosekin, Anton V. Brusnichkin, Andrei Y. Luk'yanov, Sergei A. Eremin, and Mikhail A. Proskurnin, "Heterogeneous Thermal-Lens Immunoassay for Small Organic Compounds: Determination of 4-Aminophenol," Appl. Spectrosc. 64, 942-948 (2010)
http://www.opticsinfobase.org/as/abstract.cfm?URI=as-64-8-942


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