OSA's Digital Library

Applied Spectroscopy

Applied Spectroscopy

| PUBLISHED BY SAS — AVAILABLE FROM SAS AND OSA

  • Vol. 65, Iss. 8 — Aug. 1, 2011
  • pp: 844–848

Noninvasive Subsurface Analysis Using Multiple Miniaturized Raman Probes, Part I: Basic Study of Thin-Layered Transparent Models of Biomedical Tissues

Yuko S. Yamamoto, Hideyuki Shinzawa, Yuji Matsuura, Yukihiro Ozaki, and Hidetoshi Sato

Applied Spectroscopy, Vol. 65, Issue 8, pp. 844-848 (2011)


View Full Text Article

Acrobat PDF (396 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations
  • Export Citation/Save Click for help

Abstract

This study describes a basic theory for reconstructing pure Raman signals of materials composing a multilayer sample from Raman spectra obtained using two types of miniaturized Raman probes. An illustrative example is demonstrated using a multilayer system of samples composed of the transparent plastics polymethylmethacrylate (PMMA) and polyethylene (PE) as a model of thin-layered biomedical tissues. When the same region of an object is measured using Raman probes with different focal properties, the Raman spectra provide different depth profile information depending on the level of light penetration. Thus, a detailed comparison of the spectra can provide an interesting opportunity to probe the differences between the layers. A simple analytic form is presented for reconstructing the pure Raman spectra of the embedded layer. The method applies an understanding of the Raman sampling volume in layered transparent materials to the interpretation of Raman spectra experimentally measured by multiple probes. The basic theory described here is necessary for the expansion of the technique to turbid media, such as biological samples, where light-scattering effects must be considered. The potential applications of the proposed method include material and catalyst subsurface probing through different embedded materials, such as assessment of silicon wafers, effective noninvasive screening for catalyst synthesis, and biomedical tissue research.

Virtual Issues
Vol. 6, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Yuko S. Yamamoto, Hideyuki Shinzawa, Yuji Matsuura, Yukihiro Ozaki, and Hidetoshi Sato, "Noninvasive Subsurface Analysis Using Multiple Miniaturized Raman Probes, Part I: Basic Study of Thin-Layered Transparent Models of Biomedical Tissues," Appl. Spectrosc. 65, 844-848 (2011)
http://www.opticsinfobase.org/as/abstract.cfm?URI=as-65-8-844


Sort:  Journal  |  Reset

References

References are not available for this paper.

Cited By

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited