OSA's Digital Library

Applied Spectroscopy

Applied Spectroscopy

| PUBLISHED BY SAS — AVAILABLE FROM SAS AND OSA

  • Vol. 66, Iss. 1 — Jan. 1, 2012
  • pp: 60–65

Linear Discriminant Analysis of Single-Cell Fluorescence Excitation Spectra of Five Phytoplankton Species

LAURA S BRUCKMAN, TAMMI L RICHARDSON, JOSEPH A SWANSTROM, KATHLEEN A DONALDSON, MICHAEL ALLORA, TIMOTHY J SHAW, and MICHAEL L MYRICK

Applied Spectroscopy, Vol. 66, Issue 1, pp. 60-65 (2012)


View Full Text Article

Acrobat PDF (1077 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations
  • Export Citation/Save Click for help

Abstract

Linear discriminant analysis (LDA) of single-cell fluorescence excitation spectra (λem = 680 nm) for five species of marine phytoplankton was used to determine whether intra-species variation among single cells precluded discrimination among species. Single-cell spectra were recorded in an optical trap with a custom-built spectral fluorometer. For nitrogen (N)-replete cells, separation of all five species ( Emiliania huxleyi, a coccolithophore, Thalassiosira pseudonana, a diatom, Dunaliella tertiolecta, a chlorophyte, Amphidinium carterae, a dinoflagellate, and Rhodomonas salina, a cryptophyte) was possible using only a portion of the excitation spectra (570-610 nm). This wavelength region gave perfect classification of species with a minimum Fisher ratio of 62. For four species ( E. huxleyi, T. pseudonana, D. tertiolecta, and A. carterae), variations in fluorescence excitation spectra as cells were starved of N did not impact the classification process adversely within the chosen spectral window. R. salina cells grown with and without N showed significant differences in their fluorescence excitation spectra but could still be classified if a different spectral window (490-570 nm) was used. Overall, we conclude that intra-species variation among single-cell fluorescence excitation spectra does not preclude discrimination among species.

Virtual Issues
Vol. 7, Iss. 3 Virtual Journal for Biomedical Optics

Citation
LAURA S BRUCKMAN, TAMMI L RICHARDSON, JOSEPH A SWANSTROM, KATHLEEN A DONALDSON, MICHAEL ALLORA, TIMOTHY J SHAW, and MICHAEL L MYRICK, "Linear Discriminant Analysis of Single-Cell Fluorescence Excitation Spectra of Five Phytoplankton Species," Appl. Spectrosc. 66, 60-65 (2012)
http://www.opticsinfobase.org/as/abstract.cfm?URI=as-66-1-60

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited